Species:

Universal

Alternative Name:

Prostaglandin E1; PGE1

Assay Type:

Competitive

unit:

pg/mL

Standard:

2000 pg/mL

Sensitivity:

9.55 pg/mL

Detection Range:

31.25-2000 pg/mL

Assay Time:

1.5H

Sample Type:

Cell culture supernatant, Plasma, Saliva, Serum, Urine

Research:

Immunology

Test Principle:

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with PGE1. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to PGE1. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PGE1 in the samples is then determined by comparing the OD of the samples to the standard curve.